We offer target and biomarker screening services for industry and academics providing you with cell lines expressing your target gene of choice. So far, we have generated

• 250 cDNA expression vectors,
• 850 stable recombinant cell lines for the expression of
• 200 targets, using
• 50 maternal cell lines or primary cells.
  

Target validation

We support your target validation by:
• Cloning your gene of interest into a suitable expression vector
  
• Selection of promoters of different strengths
• Lentiviral transduction of a cell line / primary cell type of your choice
• Selection of stable recombinant cell lines (polyclonal or monoclonal), validated by gene expression analysis at the mRNA / protein level
• Characterisation with respect to target protein localisation by immunofluorescence microscopy or FACS analysis
• Full range of functional characterisations (e.g. cell proliferation, induction of apoptosis, metabolism; please inquire for further assays)

High content analysis of cellular phenotype
Combine the Chromobody® technology with the expression of your gene of interest!

• Double expression of your gene of interest and of an indicative Chromobody® in a single cell line
• High content analysis for monitoring cell cycle progression, nuclear morphology, cytoskeletal integrity
  

For further information and pricing please contact
Dr. Frank Weise